Biology (AKU-BIO)
Topic 3 of 7Aga Khan Board

Genetics & Inheritance

DNA, genes, alleles, Mendelian inheritance and genetic crosses

DNA (deoxyribonucleic acid) is the molecule of inheritance. It is a double helix made of nucleotides (base + sugar + phosphate). The four bases are: A (adenine), T (thymine), G (guanine), C (cytosine). A pairs with T; G pairs with C.


A gene is a section of DNA that codes for a protein. Humans have approximately 20,000 genes on 23 pairs of chromosomes (46 total). Alleles are different versions of a gene.


Dominant allele (capital letter) is expressed when present. Recessive allele (lowercase) is only expressed when two copies are present (homozygous recessive).


Genetic crosses using Punnett squares:

  • Homozygous dominant (BB) × Homozygous recessive (bb) → all Bb (carriers, all show dominant trait)
  • Heterozygous (Bb) × Heterozygous (Bb) → BB:Bb:Bb:bb = 3 dominant : 1 recessive

  • Sex determination: Females = XX, Males = XY. Father's sperm (X or Y) determines sex.


    Genetic disorders:

  • Sickle cell anaemia: recessive (ss). Common in populations from malaria-endemic areas including Pakistan.
  • Down's syndrome: extra chromosome 21 (trisomy 21) — caused by non-disjunction during meiosis.
  • Key Points to Remember

    • 1DNA: double helix, A-T and G-C base pairs
    • 2Dominant expressed over recessive; recessive needs two copies
    • 3Punnett square: Bb × Bb gives 3:1 ratio
    • 4Father determines sex (X or Y sperm)

    Pakistan Example

    Thalassaemia — Pakistan's Genetic Crisis

    Pakistan has one of the highest rates of thalassaemia (a blood disorder) in the world — approximately 9 million carriers. Thalassaemia major occurs when a child inherits the recessive allele (tt) from both carrier parents (Tt × Tt). This gives a 25% chance for each pregnancy. AKU-EB Biology Punnett square questions regularly use thalassaemia as the Pakistani context.

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